Engineering Strategic Biofuels Solutions

Engineering Strategic Biofuels Solutions

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Description: Biofuels Market: Ethanol Production Goal, 60 Billion Gallons/year by 2030, 1/3 of U.S. gasoline market, Energy Independence & Security. Act of 2007 target 36 Billion gallons by 2022.

Economic impact: $150+ Billion direct impact. 2009 current state of ethanol industry: 219 production facilities 14 Billion gallon capacity, 2009 projected production 11.1 Billion gallons, 1998 1.47 Billion gallons 10x growth in 10 years.

Author: Mike Neibler (Fellow) | Visits: 2697 | Page Views: 2705
Domain:  Green Tech Category: Biomass 
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Xylogenics, Inc Xylogenics Inc.
Engineering St t i Bi F l S l ti E i i Strategic BioFuels Solutions
Mike Neibler, CEO 317-697-7514 www xylogenics com

Biofuels Market
Ethanol Production Goal �
60 Billion Gallons/year by 2030 y y 1/3 of U.S. gasoline market

Energy Independence & Security Act of 2007 target �
36 Billion gallons by 2022

Economic impact
� $150+ Billion direct impact

2009 current state of ethanol industry
� 219 production facilities � 14 Billion gallon capacity � 2009 projected production � 11.1 Billion gallons � 1998 � 1.47 Billion gallons 10x growth in 10 years


Ethanol Market
Energy Independence & Security Act of 2007 Ethanol Production Targets � 36 Billion Gallons by 2022


Billions of Gallons f

1st Generation

� Corn Ethanol

219 plants in the US (12 in Indiana) Issues: � Established ethanol industry infrastructure � Xylogenics 3 � 5 % yield improvement � Transition to 2nd generation cellulosic ethanol

Xylogenics Ethanol Yield Improvements


2nd Generation � Cellulosic Ethanol
� 14 pilot scale p p plants under construction in U.S. � Doesn't compete with food sources � More land available for cellulosic feedstocks � Cellulosic feedstocks � corn cobs, corn stover, switchgrass, wheat straw, barley straw, miscanthus grass, wood waste � Xylogenics 30 � 50% yield improvement.


Why ethanol
Reduces Dependence on foreign oil � 65%
I Import costs - $250 $500 Billion per year t t $250-$500 Billi

Petroleum cost going up � less light sweet crude Electric vehicles don't meet short term need - 33 years y to replace all vehicles Infrastructure in place for ethanol � blend with gasoline using same pumps E10 E15 E85 pumps, E10, E15, Reduces greenhouse gas emissions by 55 � 95% Renewable energy source Energy gain
Corn ethanol improved from 1.25 in 2005 to 1.6 to 2.2 in 2009. Cellulosic ethanol is > 4.0

Market Influencers
Public Policy
E Energy Independence & S I d d Security A t of 2007 it Act f EPA guidelines for Greenhouse Gas Emissions Energy Stimulus legislation

Land Use � land management Environmental Impact Feedstock Development & Production
Crop suitability, availability and yields

Ethanol Manufacturing Process M k t acceptance Market t


How to help ethanol market
Increase feedstock acreage
C Corn yield i i ld improvement -160 bushels / t 160 b h l /acre t 300 within 5 to ithi years Other biomass with higher biomass content per acre � switchgrass, miscanthus, switchgrass miscanthus sorghum Bioengineered crop strains

Improve ethanol production efficiency in each step
Bi Biomass pretreat chemistry t t h i t Hydrolysis chemistry Fermentation chemistry


IU Research Benefits
Research areas:
chemistry, analytical chemistry, molecular biology, yeast genetics

Critical knowledge:
cell bi l ll biology, systems biology, Metabolomics, proteomics


Indiana biofuels investment
Ethanol production � No. 3 in U.S. I di Indiana produces 10% of th ethanol i th U S about d f the th l in the U.S. b t 1 Billion gallons Corn production � No. 4 p Soybean production � No. 4 Active project to increase E85 pumps � $20K subsidy 2nd i number of E85 pumps - 120 in b f


Xylogenics History
IU School of Medicine Invention
� 2003 � filed disco er with Indiana Uni for bioengineered yeast that discovery ith Univ east improves ethanol yields (Dr. Mark Goebl, Professor Biochemistry and Molecular Biology, Indianapolis, IN genomics lab) � Lugar Center for Renewable Energy Executive Committee � May 2008 � received grant from Lugar Center for Renewable Energy � Sep 2008 � Neibler & Mercer initiate commercialization effort � Nov 2008 � formed commercialization company � Xylogenics, Inc. � Dec 2008 - funding from IU Medical Group Foundation � Feb 2009 � patent filed - 22 yeast strains and mechanism eb 009 pate t ed st a s a d ec a s � May 2009 � license exclusive rights from IU


Xylogenics Biofuels focus
� Improve process efficiencies through bioengineered solutions for 1st, 2nd, 3rd generation biofuels and beyond. � Yield Improvement � 30-50% in 2nd generation cellulosic ethanol � Oth benefit � i Other b fit improves overall f ll fermentation t ti process


Xylogenics Technology
1st Generation Corn Ethanol
Enzymes Enzymes

Glucose preference turned off

Traditional Yeast


Maltose & Tri maltose Tri-maltose

Xylogenics Yeast

Ethanol 3- 5% more Ethanol Xylose converted to Ethanol Ethanol

2nd Generation Cellulosic Ethanol

Glucose & Xylose

Traditional Yeast


Xylogenics Yeast

30- 50% more Ethanol


Xylogenics benefits for corn ethanol
� Reduce enzyme requirement by 50% o Save $600K to $1.2 Million per year � Reduce fermentation cycle time 6-8 hrs o Increases plant capacity by 15-20% � Convert additional sugars to ethanol o Increase ethanol yield 3 5% 3-5% Estimated benefit - $3 5 Million to bottom line (40MMgal) $3.5 Add 2nd generation cellulosic feedstream

Development Timeline
Proof of Concept (completed) 12 months Commercial Launch


Xylogenics Value Proposition
Ability to bioengineer yeasts or enzymes based on understanding of cell biology matched to functional requirements and environment for various biomass conversions to biofuels. Team
o Dr. Mark Goebl, professor Biochemistry & Molecular Biology � 20+ 20 year yeast geneticist and cell bi l i t t ti i t d ll biologist o Josh Heyen � PhD research � 8 years, cell growth & division regulation o Dr. Ross Cocklin � PhD research � 8 years, nutritional impacts on cell growth